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J Sep Sci. 2005 Nov;28(17):2370-3.

Determination of some polycyclic aromatic hydrocarbons in filter tar of Turkish cigarettes.

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Department of Chemistry, Sciences and Arts Faculty, Ondokuz Mayis University, Samsun, Turkey.


A simultaneous determination method for the analysis of benzo[a]pyrene (BaP) and dibenzo[a,h]anthracene (DahA) in the filter tar of Turkish cigarettes has been developed. The method involved (a) the extraction of BaP and DahA with n-hexane from ACN solution in which the cigarette filters were extracted, and then (b) purification of the n-hexane extracts by elution on an XAD-2 column using n-hexane/dichloromethane (9:1, v/v) mixture. Separation and quantitative determination of BaP and DahA in the extracts were carried out by HPLC and fluorescence detection on a C18 RP column. The calculated recoveries for BaP and DahA were found in the range of 90-100% for each extraction and clean-up steps. Analysis of various filter tar of Turkish cigarettes showed that an average of 74.28 ng/filter of BaP and 5.24 ng/filter of DahA were present in Turkish cigarettes.

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