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Eur J Clin Microbiol Infect Dis. 2005 Dec;24(12):842-5.

Real-time PCR for detection of Brucella spp. DNA in human serum samples.

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1
Laboratoire de Bactériologie, Centre Hospitalier Universitaire, Faculté de Médecine, Université Joseph Fourier, BP 217, 38043, Grenoble cedex 9, France.

Abstract

Presented here are the results of an evaluation of an in-house real-time PCR assay for the rapid and specific diagnosis of human brucellosis. The assay was based on direct amplification from serum samples of a 169-bp portion of bcsp31, a gene found in all Brucella species and biovars. Species specificity and selectivity of this real-time PCR assay were evaluated using genomic DNA from 15 Brucella strains and 42 non-Brucella strains, and the results were 100%. Among 17 culture-proven brucellosis patients, sera from 11 gave a positive amplification signal, corresponding to a sensitivity of 64.7%. In contrast, negative results were obtained for all sera from 60 control patients, corresponding to a specificity of 100%. The results indicate this test is well adapted for definite confirmation of brucellosis cases, when Brucella cultures remain sterile and serological tests demonstrate the presence of cross-reacting antibodies against Brucella sp. and Yersinia enterocolitica O:9 antigens.

PMID:
16341519
DOI:
10.1007/s10096-005-0064-0
[Indexed for MEDLINE]

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