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Methods Enzymol. 2005;399:777-99.

Ubiquitin fusion technique and related methods.

Author information

1
Division of Biology, California, Institute of Technology, Pasadena, California, USA.

Abstract

The ubiquitin fusion technique, developed in 1986, is still the method of choice for producing a desired N-terminal residue in a protein of interest in vivo. This technique is also used as a tool for protein expression. Over the past two decades, several otherwise unrelated methods were invented that have in common the use of ubiquitin fusions as a component of design. I describe the original ubiquitin fusion technique, its current applications, and other methods that use the properties of ubiquitin fusions.

PMID:
16338395
DOI:
10.1016/S0076-6879(05)99051-4
[Indexed for MEDLINE]

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