Format

Send to

Choose Destination
J Proteome Res. 2005 Nov-Dec;4(6):2148-53.

Unbiased examination of changes in plasma membrane proteins in drug resistant cancer cells.

Author information

1
Department of Chemistry and Biochemistry, University of Maryland, College Park Maryland, USA.

Abstract

In this study, an unbiased examination is made of the abundance changes between proteins found in the basolateral plasma membranes of a drug susceptible parental MCF-7 breast cancer cell line and a cell line selected from the parent line for resistance to the anticancer drug mitoxantrone. Plasma membrane proteins were differentially labeled metabolically, enriched using the colloidal silica pellicle method, and characterized by tandem mass spectrometry. Fifteen proteins were identified with significant (>2) changes, including receptors, adhesion proteins, proteins involved in amino acid uptake, and proteins involved in glucose uptake. From 40 mug of membrane proteins, 3227 unique peptides and 540 proteins were identified.

PMID:
16335961
DOI:
10.1021/pr0502370
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for American Chemical Society
Loading ...
Support Center