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J Proteome Res. 2005 Nov-Dec;4(6):1952-60.

Comparison of normal and breast cancer cell lines using proteome, genome, and interactome data.

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UCSF Comprehensive Cancer Center, University of California-San Francisco, California 94143-0808, USA.


Normal and cancer cell line proteomes were profiled using high throughput mass spectrometry techniques. Application of protein-level and peptide-level sample fractionation combined with LC-MS/MS analysis enabled identification of 2235 unmodified proteins representing a broad range of functional and compartmental classes. An iterative multistep search strategy was used to identify post-translational modifications, revealing several proteins that are preferentially modified in cancer cells. Information regarding both unmodified and modified protein forms was combined with publicly available gene expression and protein-protein interaction data. The resulting integrated dataset revealed several functionally related proteins that are differentially regulated between normal and cancer cell lines.

[Indexed for MEDLINE]

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