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[Study on the frequency of alpha-1,2-fucosyltransferase gene h4 allele (C35T) in Chinese population].

[Article in Chinese]

Author information

1
Institute of Transfusion Medicine, Blood Center of Zhejiang Province, P. R. China.

Abstract

OBJECTIVE:

To investigate the h4 allele (C35T) frequency of alpha-1,2-fucosyltransferase gene in Chinese population.

METHODS:

The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for identifying C35T variant was established by using PCR to amplify a 125 bp FUT1 gene fragment, including C35T variant sequence, and the PCR product was digested by Hae III restriction enzyme. One hundred and fifty-eight random samples from Chinese blood donor were screened by PCR-RFLP.

RESULTS:

Among 158 Chinese individuals with normal ABO and H blood group phenotypes, 8 and 83 were homozygous with 35T/T and 35C/C, respectively, while 67 were heterozygous with 35C/T. The allele frequencies were compatible with Hardy-Weinberg equilibrium.

CONCLUSION:

The C35T substitution of FUT1 gene is not a mutation which gives rise to a non-functional h allele responsible for para-Bombay phenotype but a single nucleotide polymorphism in Chinese population.

PMID:
16331565
[Indexed for MEDLINE]

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