The title compound, bis[(-)-N-(trans-4-isopropylcyclohexanecarbonyl)-D-phenylalanine] hydronium chloride, 2C19H27NO3.H3O+.Cl-, at 110 K contains four conformationally dissimilar moieties in the asymmetric unit, which are seemingly necessary in order to optimize the supramolecular association. The organic molecule forms O-H...O hydrogen-bonded carboxylic acid dimers, which are paired into six-component clusters via N-H...Cl hydrogen bonds through the two bridging chloride anions. These combined hexameric aggregates are further interlinked into extended two-dimensional network arrays via the hydronium ions by O-H...O and O-H...Cl hydrogen bonds. This study represents the first crystallographic report of nateglinide.