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Biochim Biophys Acta. 2005 Dec 20;1731(3):154-9. Epub 2005 Nov 2.

ISL1 physically interacts with BETA2 to promote insulin gene transcriptional synergy in non-beta cells.

Author information

1
Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University, Beijing 100083, China.

Abstract

ISL1 is a LIM homeodomain protein that plays an important role in insulin gene transcriptional activation and islet cell formation. BETA2 is a transcription factor in the basic helix-loop-helix (bHLH) family, which activates expression of tissue-specific genes in several developmental systems. In this study, we investigated the functional and physical interactions of ISL1 and BETA2 in promoting insulin gene transcription in non-beta cells. Using the luciferase assay, we demonstrated that ISL1 and BETA2 could activate insulin gene transcription synergistically. Co-immunoprecipitation also supported that ISL1 and BETA2 appear in one complex and this physical interaction mediates the synergy between these two proteins.

PMID:
16321656
DOI:
10.1016/j.bbaexp.2005.08.013
[Indexed for MEDLINE]

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