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Emerg Infect Dis. 2005 Oct;11(10):1578-83.

Botulinum neurotoxin detection and differentiation by mass spectrometry.

Author information

1
Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA. jbarr@cdc.gov

Abstract

Botulinum neurotoxins (BoNTs) are proteases that cleave specific cellular proteins essential for neurotransmitter release. Seven BoNT serotypes (A-G) exist; 4 usually cause human botulism (A, B, E, and F). We developed a rapid, mass spectrometry-based method (Endopep-MS) to detect and differentiate active BoNTs A, B, E, and F. This method uses the highly specific protease activity of the toxins with target peptides specific for each toxin serotype. The product peptides derived from the endopeptidase activities of BoNTs are detected by matrix-assisted laser-desorption ionization time-of-flight mass spectrometry. In buffer, this method can detect toxin equivalents of as little as 0.01 mouse lethal dose (MLD)50 and concentrations as low as 0.62 MLD50/mL. A high-performance liquid chromatography-tandem mass spectrometry method for quantifying active toxin, where the amount of toxin can be correlated to the amount of product peptides, is also described.

PMID:
16318699
PMCID:
PMC3366733
DOI:
10.3201/eid1110.041279
[Indexed for MEDLINE]
Free PMC Article
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