Format

Send to

Choose Destination
See comment in PubMed Commons below
Anticancer Res. 2005 Nov-Dec;25(6B):3893-8.

Antitumor effect of double immunization of mice with mucin 1 and its coding DNA.

Author information

  • 1Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, RAS, Miklukho-Maklaya St, 16/10, Moscow, 117997, Russian Federation.

Abstract

OBJECTIVE:

To evaluate the antitumor effect of mouse immunization with human mucin 1 gene (muc 1) DNA plasmids combined with simultaneous injections of human mucin 1 (MUC1) protein.

MATERIALS AND METHODS:

MUC1 DNA was cloned in pBK-CMV to prepare DNA plasmids and in pET22b(+) to produce proteins. Three strains of mice, immunized with DNA or DNA plus MUC1, were inoculated with tumor cells obtained from spontaneous tumors. IgG(2a) production, MUC1-specific IFN-delta-producing CD8+ T cells, tumor growth and mouse survival were monitored.

RESULTS:

Only immunization with DNA plus proteins induced IgG(2a) and intracellular IFN-delta production by CD8+ T cells in the strains tested. DNA plus protein immunization induced a better mouse survival in comparison with the DNA groups. However, all immunized mice invariably developed tumors.

CONCLUSION:

Immunization with DNA plus proteins induced a better protection from tumor growth than immunization with naked DNA. However, the efficacy of immunization with MUC1-based antigens remains low.

PMID:
16309176
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire
    Loading ...
    Support Center