(A–D) IFN-γ-induced astrocytes were infected with T. gondii for 2 h, fixed, and stained for IGTP (A), GTPI (B), TGTP1 (C), or IRG-47 (D).
(E) IFN-γ-induced astrocytes were infected with T. gondii for 2 h, fixed, and stained for IIGP1 (red) and T. gondii (green).
(F) IFN-γ-induced cells were infected with T. gondii, fixed 24 h later, and stained for IIGP1. White arrowheads point to PVs containing replicating parasites.
(G and H) IFN-γ-induced cells were infected with heat-killed (G) or live (H) parasites, fixed 2 h later, and stained for IIGP1 (green) and LAMP1 (red). White arrowheads in (G) point to parasites residing in a LAMP1-positive but IIGP1-negative compartment. (H) Shows single sections of a 3D deconvoluted Z-series. Nuclei of host cells and parasites were stained with DAPI.