Format

Send to

Choose Destination
Rapid Commun Mass Spectrom. 2005;19(24):3701-4.

Electrospray-assisted laser desorption/ionization mass spectrometry for direct ambient analysis of solids.

Author information

1
Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 80424 Taiwan. jetea@mail.nsysu.edu.tw

Abstract

A new method of electrospray-assisted laser desorption/ionization (ELDI) mass spectrometry, which combines laser desorption with post-ionization by electrospray, was applied to rapid analysis of solid materials under ambient conditions. Analytes were desorbed from solid metallic and insulating substrata using a pulsed nitrogen laser. Post-ionization produced high-quality mass spectra characteristic of electrospray, including protein multiple charging. For the first time, mass spectra of intact proteins were obtained using laser desorption without adding a matrix. Bovine cytochrome c and an illicit drug containing methaqualone were chosen in this study to demonstrate the applicability of ELDI to the analysis of proteins and synthetic organic compounds.

PMID:
16299699
DOI:
10.1002/rcm.2243

LinkOut - more resources

Full Text Sources

Other Literature Sources

Supplemental Content

Loading ...
Support Center