Format

Send to

Choose Destination
Nat Methods. 2005 Dec;2(12):955-7. Epub 2005 Nov 18.

Positional proteomics: selective recovery and analysis of N-terminal proteolytic peptides.

Author information

1
Protein Function Group, Faculty of Veterinary Science, University of Liverpool, Crown Street, Liverpool L69 7ZJ, UK.

Abstract

Bottom-up proteomics is the analysis of peptides derived from single proteins or protein mixtures, and because each protein generates tens of peptides, there is scope for controlled reduction in complexity. We report here a new strategy for selective isolation of the N-terminal peptides of a protein mixture, yielding positionally defined peptides. The method is tolerant of several fragmentation methods, and the databases that must be searched are substantially less complex.

PMID:
16299481
DOI:
10.1038/nmeth811
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center