Cloning and primary structure of the wide-spectrum amidase from Brevibacterium sp. R312: high homology to the amiE product from Pseudomonas aeruginosa

F Soubrier; S Lévy-Schil; J F Mayaux; D Pétré; A Arnaud; J Crouzet

doi:10.1016/0378-1119(92)90635-3

Cloning and primary structure of the wide-spectrum amidase from Brevibacterium sp. R312: high homology to the amiE product from Pseudomonas aeruginosa

Gene. 1992 Jul 1;116(1):99-104. doi: 10.1016/0378-1119(92)90635-3.

Authors

F Soubrier¹, S Lévy-Schil, J F Mayaux, D Pétré, A Arnaud, J Crouzet

Affiliation

¹ Département Biotechnologie, Rhône-Poulenc Rorer, Centre de Recherche de Vitry-Alfortville, Vitry sur Seine, France.

PMID: 1628849
DOI: 10.1016/0378-1119(92)90635-3

Abstract

A Brevibacterium sp. R312 DNA fragment encoding the wide-spectrum amidase (EC 3.5.1.4) has been cloned and sequenced, using limited amino acid (aa) sequence information obtained from the purified enzyme. The deduced aa sequence showed more than 80% strict identity with the Pseudomonas aeruginosa aliphatic amidase, the product of the amiE gene, suggesting a horizontal transfer of the gene during evolution between Gram+ and Gram- bacteria.

MeSH terms

Amidohydrolases / chemistry
Amidohydrolases / genetics*
Amidohydrolases / metabolism
Amino Acid Sequence
Base Sequence
Brevibacterium / enzymology*
Brevibacterium / genetics
Cloning, Molecular
Escherichia coli / enzymology
Escherichia coli / genetics*
Molecular Sequence Data
Pseudomonas aeruginosa / enzymology*
Pseudomonas aeruginosa / genetics
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Restriction Mapping

Substances

Recombinant Proteins
Amidohydrolases
amidase

Associated data

GENBANK/M74890
GENBANK/M74891
GENBANK/M74892
GENBANK/M76451
GENBANK/M77131
GENBANK/M77132
GENBANK/M79309
GENBANK/M79310
GENBANK/S38798
GENBANK/S38799