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J Med Microbiol. 2005 Dec;54(Pt 12):1183-7.

Rapid and simple detection of blaCTX-M genes by multiplex PCR assay.

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Health Protection Agency West Midlands Public Health Laboratory, Birmingham Heartlands & Solihull NHS Trust, Birmingham B9 5SS, UK.


A novel multiplex PCR assay is described (CTX-Mplex PCR) that allows rapid detection of bla(CTX-M) genes and discrimination between groups 1, 2, 9 and 25/26. The specificity and sensitivity of the assay were evaluated with 10 control strains and then applied to 62 clinical isolates. The multiplex PCR detected and classified bla(CTX-M) genes with 100 % accuracy. The utilization of a denaturing HPLC WAVE system to size the PCR products automatically from the multiplex PCR enhances the assay by saving time and costs.

[Indexed for MEDLINE]

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