Format

Send to

Choose Destination
J Med Microbiol. 2005 Dec;54(Pt 12):1183-7.

Rapid and simple detection of blaCTX-M genes by multiplex PCR assay.

Author information

1
Health Protection Agency West Midlands Public Health Laboratory, Birmingham Heartlands & Solihull NHS Trust, Birmingham B9 5SS, UK. li.xu@heartofengland.nhs.uk

Abstract

A novel multiplex PCR assay is described (CTX-Mplex PCR) that allows rapid detection of bla(CTX-M) genes and discrimination between groups 1, 2, 9 and 25/26. The specificity and sensitivity of the assay were evaluated with 10 control strains and then applied to 62 clinical isolates. The multiplex PCR detected and classified bla(CTX-M) genes with 100 % accuracy. The utilization of a denaturing HPLC WAVE system to size the PCR products automatically from the multiplex PCR enhances the assay by saving time and costs.

PMID:
16278432
DOI:
10.1099/jmm.0.46160-0
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Ingenta plc
Loading ...
Support Center