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In Vivo. 2005 Nov-Dec;19(6):989-95.

Effects of probiotic bacteria (VSL#3) on the polyamine biosynthesis and cell proliferation of normal colonic mucosa of rats.

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1
Laboratory of Biochemistry, National Institute for Digestive Diseases IRCCS "Saverio de Bellis", Castellana G., Bari, University of Bari, Italy. irccsbiochimica@libero.it

Abstract

BACKGROUND:

Probiotics seem to possess tumour inhibitory properties, but few studies have investigated their actions on the cell proliferation of normal colonic mucosa. The effects of a probiotic mixture (VSL#3) on polyamine biosynthesis, Ki-67 levels and apoptosis in the normal colon of rats were studied.

MATERIALS AND METHODS:

For a 4-week period, 20 rats were fed a VSL#3 solution and 20 rats a saline solution. Samples from the colonic mucosa were collected at the end of treatment. Polyamines were detected by HPLC, ornithine decarboxylase activity by a radiometric technique, and apoptosis and Ki-67 by histochemical and immunohistochemical methods.

RESULTS:

VSL#3 caused a significant decrease in colonic polyamine levels, ornithine decarboxylase activity and Ki-67 compared to controls. A significant increase in the apoptotic index was also observed.

CONCLUSION:

Probiotics could also reduce proliferation rates in a condition not affected by hyperproliferative or neoplastic growth, when the normal control mechanisms are still completely effective.

PMID:
16277012
[Indexed for MEDLINE]
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