Send to

Choose Destination
Biochemistry. 1992 Jul 7;31(26):6152-65.

The broad diversity of neutral and sialylated oligosaccharides derived from human salivary mucins.

Author information

Unité des Protéines, Institut National de la Santé et de la Recherche Médicale No. 16, Lille, France.


Mucin glycopeptides were prepared from the salivary mucins of 20 healthy donors with blood group O. The carbohydrate chains of the high-molecular-weight mucins were released by alkaline borohydride treatment. Neutral and monosialylated oligosaccharide-alditols were purified by ion-exchange chromatography, gel filtration, and HPLC. The structures of the oligosaccharide-alditols were determined by high-resolution 1H-NMR spectroscopy in combination with fast atom bombardment mass spectrometry and methylation analysis. Thirty-seven oligosaccharide-alditols were characterized and illustrate the extreme diversity of the salivary mucins carbohydrate chains. This diversity might represent a mosaic of bacterial adhesion sites and be involved in the early events of the nonimmune defense of the oral cavity. Among these 37 oligosaccharide-alditols, 31 have not been previously described in human saliva and five of these are novel structures: [formula: see text].

[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center