Format

Send to

Choose Destination
Methods Enzymol. 2005;398:103-12.

Expression and evaluation of RING finger proteins.

Author information

1
Dynamics and Signaling, National Cancer Institute at Frederick, Frederick, MD 21702, USA.

Abstract

RING finger proteins represent the largest class of potential ubiquitin ligases. This chapter describes methods used to express and assess the activity of proteins containing RING fingers based on our experience with a number of different family members. In addition to general protocols for assessing activity, specific protocols are provided for evaluating the ubiquitylation of p53 by the RING finger E3 Hdm2/Mdm2. Use of these methods may help identify new E3s, dissect factors involved in ubiquitylation of substrates, and screen for molecules that affect ubiquitylation.

PMID:
16275323
DOI:
10.1016/S0076-6879(05)98010-5
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center