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FEMS Microbiol Lett. 2005 Dec 15;253(2):315-21. Epub 2005 Oct 14.

Synthesis of a small, cysteine-rich, 29 amino acids long peptide in Mycoplasma pneumoniae.

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Zentrum für Molekulare Biologie Heidelberg, Universität Heidelberg, 69120 Heidelberg, Germany.


A 205-210 bases long, small RNA (MP200RNA) of Mycoplasma pneumoniae encodes an open reading frame (ORF pmp200) that has the potential to be translated into a 29 amino acids long peptide with nine cysteines. The expression of this peptide in M. pneumoniae was proven indirectly by constructing a gene fusion between the ORF pmp200 and mrfp1, the gene encoding the monomeric red fluorescent protein. The fusion construct was translated in M. pneumoniae. The corresponding fusion protein, with a molecular mass of approximately 35,000 Da, was isolated and the correct sequence was proven by Edman degradation and by mass spectrometry.

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