Format

Send to

Choose Destination
Cancer Lett. 2006 Aug 18;240(1):60-8. Epub 2005 Oct 25.

An anti-tumor role for cGMP-dependent protein kinase.

Author information

1
Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, GA 30912, USA.

Abstract

This study compared Type-1 cGMP-dependent protein kinase (PKG) expression in normal and tumor tissues and examined PKG function in tumor growth. Studies with a cDNA array revealed that PKG expression was reduced in many tumors compared to respective normal tissue. This decrease in PKG expression was confirmed using quantitative RT-PCR and western blotting of matched colon specimens from normal epithelium and tumor tissue, and also in colon derived cell lines where luciferase reporter analysis revealed that the decreased expression occurred at the transcriptional level. Using SW620 colon carcinoma cells engineered for inducible expression of PKG1beta, it was found that exogenous PKG1beta lead to decreased tumor growth and invasiveness in nude mouse xenografts.

PMID:
16256267
DOI:
10.1016/j.canlet.2005.08.035
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center