nov: a new genetic locus that affects the response of Escherichia coli K-12 to novobiocin

J Rakonjac; M Milic; D Ajdic-Predic; D Santos; R Ivanisevic; D J Savic

doi:10.1111/j.1365-2958.1992.tb00876.x

nov: a new genetic locus that affects the response of Escherichia coli K-12 to novobiocin

Mol Microbiol. 1992 Jun;6(11):1547-53. doi: 10.1111/j.1365-2958.1992.tb00876.x.

Authors

J Rakonjac¹, M Milic, D Ajdic-Predic, D Santos, R Ivanisevic, D J Savic

Affiliation

¹ Institute of Molecular Genetics and Genetic Engineering, Belgrade, Yugoslavia.

PMID: 1625583
DOI: 10.1111/j.1365-2958.1992.tb00876.x

Abstract

We have identified a new gene locus (nov) affecting the resistance of Escherichia coli K-12 to novobiocin. The gene also affects, although to a lesser extent, tolerance to another gyrase inhibitor coumermycin. Transductional and complementation analysis show that nov is located between att phi 80 and the osmZ (hns) genes at minute 27 of the E. coli K-12 genetic map. In standard laboratory strains of E. coli K-12 nov exists at least in two allelic forms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Aminocoumarins
Biological Transport / genetics
Cell Membrane Permeability / genetics
Chromosome Mapping
Coumarins / pharmacology
DNA, Bacterial / genetics
DNA, Superhelical / genetics
Drug Resistance, Microbial / genetics
Escherichia coli / drug effects
Escherichia coli / genetics*
Genes, Bacterial / genetics*
Genes, Bacterial / physiology
Genes, Suppressor / physiology
Mutation
Novobiocin / metabolism
Novobiocin / pharmacology*

Substances

Aminocoumarins
Coumarins
DNA, Bacterial
DNA, Superhelical
Novobiocin
coumermycin