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Proteomics. 2005 Nov;5(16):4070-81.

High throughput production of mouse monoclonal antibodies using antigen microarrays.

Author information

1
Biochemical Instrumentation Programme, European Molecular Biology Laboratory, Heidelberg, Germany.

Abstract

Recent advances in proteomics research underscore the increasing need for high-affinity monoclonal antibodies, which are still generated with lengthy, low-throughput antibody production techniques. Here we present a semi-automated, high-throughput method of hybridoma generation and identification. Monoclonal antibodies were raised to different targets in single batch runs of 6-10 wk using multiplexed immunisations, automated fusion and cell-culture, and a novel antigen-coated microarray-screening assay. In a large-scale experiment, where eight mice were immunized with ten antigens each, we generated monoclonal antibodies against 68 of the targets (85%), within 6 wk of the primary immunization.

PMID:
16254927
DOI:
10.1002/pmic.200401279
[Indexed for MEDLINE]

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