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PLoS Genet. 2005 Oct;1(4):e55. Epub 2005 Oct 28.

Gain-of-function screen for genes that affect Drosophila muscle pattern formation.

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1
Max Planck Institut für biophysikalische Chemie, Göttingen, Germany.

Abstract

This article reports the production of an EP-element insertion library with more than 3,700 unique target sites within the Drosophila melanogaster genome and its use to systematically identify genes that affect embryonic muscle pattern formation. We designed a UAS/GAL4 system to drive GAL4-responsive expression of the EP-targeted genes in developing apodeme cells to which migrating myotubes finally attach and in an intrasegmental pattern of cells that serve myotubes as a migration substrate on their way towards the apodemes. The results suggest that misexpression of more than 1.5% of the Drosophila genes can interfere with proper myotube guidance and/or muscle attachment. In addition to factors already known to participate in these processes, we identified a number of enzymes that participate in the synthesis or modification of protein carbohydrate side chains and in Ubiquitin modifications and/or the Ubiquitin-dependent degradation of proteins, suggesting that these processes are relevant for muscle pattern formation.

PMID:
16254604
PMCID:
PMC1270011
DOI:
10.1371/journal.pgen.0010055
[Indexed for MEDLINE]
Free PMC Article
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