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Hum Reprod. 2006 Feb;21(2):436-42. Epub 2005 Oct 27.

Rejection patterns in allogeneic uterus transplantation in the mouse.

Author information

1
Department of Obstetrics and Gynaecology, Sahlgrenska Academy, Göteborg University, Göteborg, Sweden. randa.racho@obgyn.nu.se

Abstract

BACKGROUND:

Transplantation of the uterus in the mouse has been developed as a model system for research towards human uterine transplantation. Previous studies in a mouse model have demonstrated that a syngeneic uterus transplant can give rise to normal offspring. The aim of this study was to characterize the time course of rejection in a fully allogeneic mouse uterus transplantation model.

METHODS:

Uteri of BALB/c mice were transplanted to a heterotopic position in C57BL/6 recipients, whose native uteri were left in situ. The blood flow of the uteri, their gross appearance and general histology and the density of T-lymphocytes were examined on postoperative days 2-28.

RESULTS:

Macroscopic signs of rejection were apparent from day 5. At the light microscopy level, minimal inflammatory changes were seen from day 5 and massive inflammation was seen from day 10 to day 15. At day 28, necrosis and fibrosis were seen. The density of T-lymphocytes (CD3(+)) was increased in the grafted uterus from day 2 in the myometrium and from day 5 in the endometrium. Blood flow in the grafted uteri was reduced from day 15.

CONCLUSION:

A murine model to study rejection of allogeneic uterus transplants was characterized. Signs of rejection were seen from day 2 to day 5 and severe rejection was seen from day 10 to day 15. The data will be useful in future studies on immunosuppressants in this model.

PMID:
16253976
DOI:
10.1093/humrep/dei349
[Indexed for MEDLINE]

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