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Opt Lett. 2005 Oct 15;30(20):2760-2.

Spectral- and frequency-encoded fluorescence imaging.

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Harvard Medical School and the Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.


A method for obtaining fluorescence images with a high number of resolvable points by using spectral and frequency encoding is presented. Broadband excitation light is encoded with a wavelength-dependent frequency modulation and dispersed onto the sample with a grating to simultaneously illuminate an entire image line. The Fourier transform of the frequency-encoded fluorescence emission provides one line of the image. Mechanical scanning along a direction orthogonal to the wavelength-encoded axis allows creation of the two-dimensional fluorescent image. This method is applicable for developing submillimeter diameter endoscopes. The principles of the technique are validated by imaging indocyanine green fluorescence in microfluidic channels.

[Indexed for MEDLINE]

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