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Opt Lett. 2005 Oct 15;30(20):2712-4.

Laser-tweezer-controlled solid immersion microscopy in microfluidic systems.

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Department of Electrical and Computer Engineering, University of California, San Diego, La Jolla, California 92093, USA.


We describe the creation and implementation of a near-field scanning solid immersion microscope that is specifically tailored for use in microfluidic systems. The microscope comprises a newly fabricated Weierstrass solid immersion lens (SIL), which is detached from its substrate and is free floating in the fluid, and a laser optical tweezer, which serves both as a trapping beam for alignment and positioning of the SIL and as a near-field scanning beam that images the sample through the SIL. A discussion of the SIL's fabrication method is presented along with experimental results that demonstrate the effectiveness of our microscope design.

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