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Archaea. 2005 Dec;1(6):375-84.

Role of the precorrin 6-X reductase gene in cobamide biosynthesis in Methanococcus maripaludis.

Author information

1
Department of Microbiology, The Ohio State University, Columbus, OH, USA.

Abstract

In Methanococcus maripaludis strain JJ, deletion of the homolog to cbiJ, which encodes the corrin biosynthetic enzyme precorrin 6-X reductase, yielded an auxotroph that required either cobamide or acetate for good growth. This phenotype closely resembled that of JJ117, a mutant in which tandem repeats were introduced into the region immediately downstream of the homolog of cbiJ. Mutant JJ117 also produced low quantities of cobamides, about 15 nmol g(-1) protein or 1-2% of the amount found in wild-type cells. These results confirm the role of the cbiJ homolog in cobamide biosynthesis in the Archaea and suggest the presence of low amounts of a bypass activity in these organisms.

PMID:
16243778
PMCID:
PMC2685584
DOI:
10.1155/2005/903614
[Indexed for MEDLINE]
Free PMC Article

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