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FEMS Microbiol Lett. 2005 Dec 1;253(1):95-101. Epub 2005 Oct 5.

PscF is a major component of the Pseudomonas aeruginosa type III secretion needle.

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Laboratoire de Biochimie et Biophysique des Systèmes Intégrés (UMR 5092 CNRS/CEA/UJF), DRDC/BBSI, CEA Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 09, France.


Pseudomonas aeruginosa, a Gram-negative opportunistic pathogen, translocates exoenzymes (Exo) directly into the eukaryotic cell cytoplasm. This is accomplished by a type III secretion/translocation machinery. Here, we show that the P. aeruginosa type III secretory needle structure is composed essentially of PscF, a protein required for secretion and P. aeruginosa cytotoxicity. Partially purified needles, detached from the bacterial surface, are 60-80 nm in length and 7 nm in width, resembling needles from Yersinia spp.. YscF of Yersinia enterocolitica was able to functionally complement the pscF deletion, but required 11 P. aeruginosa-specific amino acids at the N-terminus for its function.

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