[Characteristics of porcine thoracic arteries fixed with polyepoxy compound]

Sichuan Da Xue Xue Bao Yi Xue Ban. 2005 Sep;36(5):692-5.
[Article in Chinese]

Abstract

Objective: To investigate the characteristics of porcine thoracic arteries fixed with ethylene glycol diglycidyl ether (EX-810) and to provide the proper scaffold materials for tissue-engineered blood vessel.

Methods: The porcine thoracic arteries were respectively treated with 40 ml/L EX-810 and 6.25 g/L glutaraldehyde, and then they were examined with naked-eye, light microscope and scanning electron microscope. The fixation index determination, the amino acid analysis and the biomechanics test were also performed.

Results: The antigenicity of vascular tissues can be diminished by EX-810 through getting rid of cell in the vascular tissues or reducing the level of free amino groups in the vascular tissues. The structural integrity of vascular tissues can be preserved after treatment with EX-810. It was also found that the EX-810-fixed porcine vascular tissues appeared more similar to the natural vascular tissues in color and mechanical properties, and were more pliable than the glutaraldehyde-fixed tissues.

Conclusion: The EX-810-fixed porcine thoracic arteries with low cytotoxicity and low antigenicity showed favorable characteristic similar to those of natural vessel, and it should be a promising material for fabricating scaffold of tissue-engineered blood vessel.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Epoxy Resins / pharmacology*
  • Swine
  • Thoracic Arteries / anatomy & histology
  • Thoracic Arteries / drug effects*
  • Tissue Engineering*
  • Tissue Fixation

Substances

  • Epoxy Resins
  • Quetol 651