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J Biosci Bioeng. 2005 Jan;99(1):38-42.

Promoter of Arabidopsis thaliana phosphate transporter gene drives root-specific expression of transgene in rice.

Author information

1
Faculty of Bioresources, Mie University, 1515 Kamihama, Tsu 514-8507, Japan.

Abstract

The PHT1 promoter::GUS fusion gene was constructed and introduced into Arabidopsis and rice by Agrobacterium-mediated transformation. Strong beta-glucuronidase (GUS) activity was detected in roots and showed phosphate starvation induction both in Arabidopsis and rice. In contrast, GUS activity in aerial tissues such as those of the leaf and stem was low. In situ GUS staining of root tissue indicated that PHT1 was expressed in root hairs and the outer layer of the main roots, but not in root tips. The PHT1 promoter has a desirable character for biotechnological transgene expression in monocot rice plants.

PMID:
16233751
DOI:
10.1263/jbb.99.038
[Indexed for MEDLINE]

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