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J Biosci Bioeng. 2003;95(3):310-2.

A novel bioconversion of L-fructose to L-glucose by Klebsiella pneumoniae.

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Department of Biochemistry and Food Science, Faculty of Agriculture and Rare Sugar Research Center, Kagawa University, Miki-cho, Kagawa 761-0795, Japan.


L-Fructose, which was produced from L-psicose using immobilized D-tagatose 3-epimerase, was utilized as a starting material in the preparation of an uncommon aldose-hexose, L-glucose, by cell reaction. A mutant strain, Klebsiella pneumoniae strain 40bXX, produced D-arabinose isomerase constitutively. Toluene-treated cells of the mutant strain, which were used as the source of crude D-arabinose isomerase, were employed in the conversion of L-fructose to L-glucose. Empirically, 0.35 g of L-glucose was obtained from 1.0 g of L-fructose, viz an overall yield of 35%. The product obtained was purified and identified to be L-glucose by high performance liquid chromatography (HPLC) analysis, and was ultimately confirmed by 13C nuclear magnetic resonance (13C NMR) spectra.


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