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Arthritis Rheum. 1992 Jul;35(7):806-11.

Type X collagen synthesis in human osteoarthritic cartilage. Indication of chondrocyte hypertrophy.

Author information

1
Max-Planck-Society, Department of Orthopaedics, Waldkrankenhaus St. Marien, Erlangen, Federal Republic of Germany.

Abstract

OBJECTIVE:

To investigate the appearance of hypertrophic chondrocytes in osteoarthritic (OA) cartilage, using type X collagen as a specific marker.

METHODS:

The biosynthesis of type X collagen was examined by metabolic labeling of freshly isolated articular chondrocytes with 3H-proline, immunoprecipitation, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the synthesized collagens. Extracellular deposition of types X and II collagen was analyzed immunohistochemically.

RESULTS:

Immunostaining revealed an irregular distribution of type X collagen, which was localized around chondrocyte clusters in fibrillated OA cartilage, but was absent from the noncalcified region of normal articular cartilage. Freshly isolated OA chondrocytes synthesized predominantly type X collagen, while control chondrocytes synthesized mostly type II collagen.

CONCLUSION:

Our findings indicate focal premature chondrocyte differentiation to hypertrophic cells in OA cartilage.

PMID:
1622419
DOI:
10.1002/art.1780350715
[Indexed for MEDLINE]

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