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Appl Environ Microbiol. 1992 May;58(5):1699-704.

Acyloin formation by benzoylformate decarboxylase from Pseudomonas putida.

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1
Department of Biology, University of Waterloo, Ontario, Canada.

Abstract

Whole cells and cell extracts of Pseudomonas putida grown in a medium containing ammonium mandelate have the capacity to produce the acyloin compound 2-hydroxypropiophenone when incubated with benzoylformate and acetaldehyde. Benzaldehyde and benzyl alcohol were formed as reaction by-products. The enantiomeric excess of the 2-hydroxypropiophenone product was found to be 91 to 92%. The absolute configuration of the enzymatically prepared product at the carbinol carbon was found to be S. The thiamine PPi-linked enzyme benzoylformate decarboxylase, purified to give a single protein band on polyacrylamide gel electrophoresis, was shown to be responsible for the catalysis of this novel condensation reaction.

PMID:
1622241
PMCID:
PMC195660
[Indexed for MEDLINE]
Free PMC Article
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