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J Am Chem Soc. 2003 Sep 17;125(37):11146-7.

Novel fluorogenic substrates for imaging beta-lactamase gene expression.

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1
Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California, Los Angeles, California 90095-1770, USA.

Abstract

A new class of small nonfluorescent fluorogenic substrates becomes brightly fluorescent after beta-lactamase hydrolysis with up to 153-fold enhancement in the fluorescence intensity. Less than 500 fM of beta-lactamase in cell lysates can be readily detected, and beta-lactamase expression in living cells can be imaged with a red fluorescence derivative. These new fluorogenic substrates should find uses in clinical diagnostics and facilitate the applications of beta-lactamase as a biosensor.

PMID:
16220906
DOI:
10.1021/ja036126o
[Indexed for MEDLINE]
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