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Biochimie. 2006 Mar-Apr;88(3-4):271-6. Epub 2005 Sep 21.

Interplay between the cis-prenyltransferases and polyprenol reductase in the yeast Saccharomyces cerevisiae.

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  • 1Institute of Biochemistry and Biophysics PAS, Pawinskiego 5a str, 02-106 Warsaw, Poland.


Dolichol formation is examined in three Saccharomyces cerevisiae strains with mutations in the ERG20 gene encoding farnesyl diphosphate synthase (mevalonic acid pathway) and/or the ERG9 gene encoding squalene synthase (sterol synthesis pathway) differing in the amount and chain length of the polyisoprenoids synthesized. Our results suggest that the activities of two yeast cis-prenyltransferases Rer2p and Srt1p and polyprenol reductase are not co-regulated and that reductase may be the rate-limiting enzyme in dolichol synthesis if the amount of polyisoprenoids synthesized exceeds a certain level. We demonstrate that reductase preferentially acts on typical polyprenols with 13-18 isoprene residues but can reduce much longer polyprenols with even 32 isoprene residues.

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