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J Infect. 2006 May;52(5):311-4. Epub 2005 Oct 6.

Prevalence of metallo-beta lactamase (MBL) producing Pseudomonas spp. and Acinetobacter spp. in a tertiary care hospital in India.

Author information

1
Department of Microbiology, Government Medical College and Hospital, 1154 A, Sec-32 B, Chandigarh 160030, India. varshagupta_99@yahoo.com

Abstract

OBJECTIVE:

Gram-negative bacteria with acquired metallo-beta lactamases production have been increasingly reported in some countries, necessitating their detection. We determined the occurrence of acquired metallo-beta lactamases (MBL) producing bacteria in our teaching hospital.

METHOD:

The method employed was a rapid technique using a disk of imipenem plus 750 microg of EDTA, on Mueller-Hinton plate which was supplemented with 70 microg/ml of zinc sulfate, to differentiate MBL producing Pseudomonads and Acinetobacter.

RESULT:

Out of 200 isolates tested, 7.5% of our Pseudomonads and Acinetobacter were shown to be MBL producers.

CONCLUSIONS:

Though MBL prevalence is still low in our hospital set up, screening for MBL among Pseudomonas and Acinetobacter isolates should be routinely done in laboratory.

PMID:
16213590
DOI:
10.1016/j.jinf.2005.08.013
[Indexed for MEDLINE]

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