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FEBS Lett. 2005 Oct 24;579(25):5626-30. Epub 2005 Sep 29.

Determinants of the nucleolar targeting of protein phosphatase-1.

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1
Division of Biochemistry, Department of Molecular Cell Biology, Faculty of Medicine, Campus Gasthuisberg, KULeuven, Belgium.

Abstract

The ubiquitously expressed protein Ser/Thr phosphatase-1 isoforms PP1alpha, PP1beta and PP1gamma1 are dynamically targeted to distinct, but overlapping cellular compartments by associated proteins. Within the nucleus of HeLa cells, EGFP-tagged PP1gamma1 and PP1beta were predominantly targeted to the nucleoli, while PP1alpha showed a more diffuse distribution. Using PP1 chimaeras and point mutants we show here that a single N-terminal residue, i.e., Gln20 for PP1alpha, Arg19 for PP1beta and Arg20 for PP1gamma1 accounts for their distinct subnuclear distribution. Our data also suggest that the N-terminus of PP1beta and PP1gamma1 harbours an interaction site for one or more nucleolar interactors.

PMID:
16213493
DOI:
10.1016/j.febslet.2005.09.033
[Indexed for MEDLINE]
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