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Clin Diagn Lab Immunol. 2005 Oct;12(10):1145-51.

Development of a microsphere-based serologic multiplexed fluorescent immunoassay and a reverse transcriptase PCR assay to detect murine norovirus 1 infection in mice.

Author information

1
Research Animal Diagnostic Laboratory, Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, E108 Veterinary Medicine Building, 1600 E. Rollins Road, Columbia, MO 65211, USA. hsuc@missouri.edu

Abstract

Murine norovirus 1 (MNV-1) is a newly recognized pathogen of mice that causes lethal infection in mice deficient in components of the innate immune response but not in wild-type 129 mice. In this study, in vitro-propagated MNV-1 was used as antigen to develop a multiplexed fluorescent immunoassay (MFI) to detect antibodies to MNV-1 in infected mice. The MNV-1 MFI was 100% specific and 100% sensitive in detecting anti-MNV-1 antibody in sera from experimentally infected mice. Testing of a large number of mouse serum samples (n = 12,639) submitted from contemporary laboratory mouse colonies in the United States and Canada revealed that 22.1% of these sera contained antibodies to MNV-1, indicating infection with MNV-1 is widespread in research mice. In addition, a reverse transcriptase PCR primer pair with a sensitivity of 25 virus copies was developed and used to demonstrate that MNV-1 RNA could be detected in the spleen, mesenteric lymph node, and jejunum from some experimentally infected mice 5 weeks postinoculation. These diagnostic assays provide the necessary tools to define the MNV-1 infection status of research mice and to aid in the establishment of laboratory mouse colonies free of MNV-1 infection.

PMID:
16210475
PMCID:
PMC1247840
DOI:
10.1128/CDLI.12.10.1145-1151.2005
[Indexed for MEDLINE]
Free PMC Article

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