Tularemia in Denmark: identification of a Francisella tularensis subsp. holarctica strain by real-time PCR and high-resolution typing by multiple-locus variable-number tandem repeat analysis

Mona Byström; Sidsel Böcher; Anna Magnusson; Jørgen Prag; Anders Johansson

doi:10.1128/JCM.43.10.5355-5358.2005

Tularemia in Denmark: identification of a Francisella tularensis subsp. holarctica strain by real-time PCR and high-resolution typing by multiple-locus variable-number tandem repeat analysis

J Clin Microbiol. 2005 Oct;43(10):5355-8. doi: 10.1128/JCM.43.10.5355-5358.2005.

Authors

Mona Byström¹, Sidsel Böcher, Anna Magnusson, Jørgen Prag, Anders Johansson

Affiliation

¹ Department of NBC-Analysis, Swedish Defence Research Agency, SE-901 82 Umeå, Sweden.

Abstract

We report ulceroglandular tularemia affecting an 8-year-old boy and the first recovery of Francisella tularensis in Denmark. A novel real-time PCR assay was used to identify the strain as F. tularensis subsp. holarctica (type B). Multiple-locus variable-number tandem repeat analysis demonstrated a close genetic relationship to strains from Norway.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques*
Base Sequence
Child
Cluster Analysis
DNA, Bacterial / analysis*
Denmark
Francisella tularensis / classification*
Francisella tularensis / genetics
Francisella tularensis / isolation & purification
Humans
Male
Minisatellite Repeats / genetics*
Molecular Sequence Data
Polymerase Chain Reaction / methods*
Tularemia / diagnosis*
Tularemia / microbiology

Substances

DNA, Bacterial