Format

Send to

Choose Destination
See comment in PubMed Commons below
J Clin Microbiol. 2005 Oct;43(10):5332-7.

Evaluation of universal probes and primer sets for assessing total bacterial load in clinical samples: general implications and practical use in endodontic antimicrobial therapy.

Author information

1
Division of Oral Microbiology and Immunology, Department of Operative and Preventive Dentistry & Peridontology, RWTH Aachen University Hospital, Aachen, Germany.

Abstract

By re-examining 10 previously published "universal" PCR assays using the ARB phylogenetic software package and database with 41,000 16S rRNA gene sequences, we found that they differed considerably in their coverage of the domain Bacteria. We evaluated the broadest-range real-time quantitative PCR protocol for its efficacy in measuring the antimicrobial effects of endodontic treatments.

PMID:
16208011
PMCID:
PMC1248440
DOI:
10.1128/JCM.43.10.5332-5337.2005
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center