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Mutat Res. 2005 Oct 15;578(1-2):15-22. Epub 2005 Apr 8.

Protection against aflatoxin-B1-induced liver mutagenesis by Scutellaria baicalensis.

Author information

1
Centre for Biomedical Research, University of Victoria, P.O. Box 3020 STN CSC, Victoria, BC, Canada V8W 3N5. jdboer@uvic.ca

Abstract

We have measured the inhibition of the mutagenicity of the mycotoxin aflatoxin-B(1) in the liver of the rat by plant material of Scutellaria baicalensis, or Huang-qin. The addition of one percent dried Huang-qin to the feed of the animals reduced the mutant frequency of a subsequent administration of aflatoxin-B1 by approximately 60 and 77%, respectively, for two different batches of the plant material. The addition of Huang-qin also increased the expression of the gene for glutathione S-transferase A5 subunit by 2.5-3.0-fold, and decreased expression of P450 cytochrome 3A2 by 1.8-2.0-fold. The greater increase of the expression of the GST gene may result in the protection shown by Huang-qin. The sensitivity of the hepatic mitochondria to swelling, a measure of the mitochondrial permeability transition, is increased significantly in animals that are on a diet containing Huang-qin. This may lead to increased sensitivity to apoptosis on treatment with toxic compounds. The two batches of Huang-qin material show differences in both chemical composition and preventive potential. This study demonstrates how a combination of generating and analysis of plant varieties together with a mammalian assay for efficacy may improve the search for better plant-based prevention of cancer initiation.

PMID:
16202794
DOI:
10.1016/j.mrfmmm.2005.01.016
[Indexed for MEDLINE]

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