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Biochim Biophys Acta. 2005 Dec 30;1754(1-2):183-90. Epub 2005 Sep 12.

Characterisation of kinase-selective inhibitors by chemical proteomics.

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Department of Molecular Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18A, 82152 Martinried, Germany.


Low-molecular-weight inhibitors of protein kinases are extensively used as research tools in signal transduction analysis and constitute a rapidly growing class of therapeutics for targeted intervention in human diseases. To determine how kinase-selective drugs interfere with cellular physiology on the molecular level, experimental strategies relying on the affinity capture of cellular targets in combination with protein identification by mass spectrometry have been established for a variety of kinase inhibitors. Importantly, these chemical proteomic methods permit the direct analysis of kinase inhibitor selectivity in biological systems and have led to new insights into the cellular modes of action of kinase-selective small molecule antagonists.

[Indexed for MEDLINE]

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