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J Microbiol Methods. 2006 May;65(2):354-6. Epub 2005 Sep 27.

Direct detection of human Staphylococcus aureus carriage in the nose using the Lightcycler Staphylococcus kit.

Author information

1
Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands.

Abstract

The Lightcycler Staphylococcus kit is a diagnostic tool for direct real-time detection of Staphylococcus aureus in clinical materials. We show here that detection of S. aureus nasal carriage using this test is hampered by competition of DNA from coagulase-negative staphylococci. However the test is well suited for species identification after culture and the identification of high-load S. aureus carriers.

PMID:
16194577
DOI:
10.1016/j.mimet.2005.08.009
[Indexed for MEDLINE]
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