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EMBO J. 2005 Oct 19;24(20):3519-31. Epub 2005 Sep 29.

Nup155 regulates nuclear envelope and nuclear pore complex formation in nematodes and vertebrates.

Author information

1
European Molecular Biology Laboratory, Heidelberg, Germany.

Abstract

Nuclear envelope (NE) formation during cell division in multicellular organisms is a central yet poorly understood biological process. We report that the conserved nucleoporin Nup155 has an essential function in NE formation in Caenorhabditis elegans embryos and in Xenopus laevis egg extracts. In vivo depletion of Nup155 led to failure of nuclear lamina formation and defects in chromosome segregation at anaphase. Nup155 depletion inhibited accumulation of nucleoporins at the nuclear periphery, including those recruited to chromatin early in NE formation. Electron microscopy analysis revealed that Nup155 is also required for the formation of a continuous nuclear membrane in vivo and in vitro. Time-course experiments indicated that Nup155 is recruited to chromatin at the time of NE sealing, suggesting that nuclear pore complex assembly has to progress to a relatively late stage before NE membrane assembly occurs.

PMID:
16193066
PMCID:
PMC1276708
DOI:
10.1038/sj.emboj.7600825
[Indexed for MEDLINE]
Free PMC Article

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