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Nat Struct Mol Biol. 2005 Oct;12(10):910-4. Epub 2005 Sep 25.

Structure of the calcium-rich signature domain of human thrombospondin-2.

Author information

1
Department of Medicine, 4285B Medical Sciences Center, University of Wisconsin-Madison, 1300 University Avenue, Madison, Wisconsin 53706, USA.

Abstract

Thrombospondins (THBSs) are secreted glycoproteins that have key roles in interactions between cells and the extracellular matrix. Here, we describe the 2.6-A-resolution crystal structure of the glycosylated signature domain of human THBS2, which includes three epidermal growth factor-like modules, 13 aspartate-rich repeats and a lectin-like module. These elements interact extensively to form three structural regions termed the stalk, wire and globe. The THBS2 signature domain is stabilized by these interactions and by a network of 30 bound Ca(2+) ions and 18 disulfide bonds. The structure suggests how genetic alterations of THBSs result in disease.

PMID:
16186819
PMCID:
PMC2219892
DOI:
10.1038/nsmb997
[Indexed for MEDLINE]
Free PMC Article

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