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Infect Immun. 2005 Oct;73(10):7022-6.

Digalactoside expression in the lipopolysaccharide of Haemophilus influenzae and its role in intravascular survival.

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1
Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, UK. r.griffin@imperial.ac.uk

Abstract

Digalactoside (galalpha-1-4 galbeta) structures of the lipopolysaccharide (LPS) of Haemophilus influenzae are implicated in virulence. A confounding factor is that tetranucleotide repeats within the lic2A, lgtC, and lex2 genes mediate phase-variable expression of the digalactosides. By deleting these repeats, we constructed recombinant strains of RM153 constitutively expressing either one or two LPS digalactosides. Expression of two digalactosides, rather than one, was associated with increased virulence of H. influenzae in vivo.

PMID:
16177385
PMCID:
PMC1230920
DOI:
10.1128/IAI.73.10.7022-7026.2005
[Indexed for MEDLINE]
Free PMC Article
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