Format

Send to

Choose Destination
Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):13855-60. Epub 2005 Sep 19.

NO-mediated cytoprotection: instant adaptation to oxidative stress in bacteria.

Author information

1
Department of Biochemistry, New York University Medical Center, New York, NY 10016, USA.

Abstract

Numerous sophisticated systems have been described that protect bacteria from increased levels of reactive oxygen species. Although indispensable during prolonged oxidative stress, these response systems depend on newly synthesized proteins, and are hence both time and energy consuming. Here, we describe an "express" cytoprotective system in Bacillus subtilis which depends on nitric oxide (NO). We show that NO immediately protects bacterial cells from reactive oxygen species by two independent mechanisms. NO transiently suppresses the enzymatic reduction of free cysteine that fuels the damaging Fenton reaction. In addition, NO directly reactivates catalase, a major antioxidant enzyme that has been inhibited in vivo by endogenous cysteine. Our data also reveal a critical role for bacterial NO-synthase in adaptation to oxidative stress associated with fast metabolic changes, and suggest a possible role for NO in defending pathogens against immune oxidative attack.

PMID:
16172391
PMCID:
PMC1236549
DOI:
10.1073/pnas.0504307102
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center