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Arch Otolaryngol Head Neck Surg. 2005 Sep;131(9):809-14.

Combined effect of hyberbaric oxygen and N-acetylcysteine on fibroblast proliferation.

Author information

1
Wound Healing and Tissue Engineering Laboratory, Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, Calif., USA.

Abstract

OBJECTIVE:

To examine the combined effect of hyperbaric oxygen and N-acetylcysteine, a well-studied antioxidant, on fibroblast proliferation and production of 3 specific growth factors: basic fibroblast growth factor, vascular endothelial growth factor, and transforming growth factor beta1.

DESIGN:

In vitro study.

SUBJECTS:

None.

INTERVENTIONS:

Human dermal fibroblasts were propagated in serum-free medium and subjected to daily 90-minute 2-atm hyperbaric oxygen treatments with varying concentrations of N-acetylcysteine for 7 consecutive days. Cell proliferation and growth factor assays were performed on days 0, 1, 3, 5, and 7.

RESULTS:

Population doubling time decreased significantly with 40 micromol/L of N-acetylcysteine supplementation of 2-atm hyperbaric oxygen treatment. Higher levels of N-acetylcysteine increased population doubling time.

CONCLUSIONS:

Supplementation of hyperbaric oxygen therapy with 40 mumol/L of N-acetylcysteine appears to increase fibroblast proliferation without producing an unfavorable growth factor profile for normal healing. This suggests that this level of N-acetylcysteine may foster an ideal redox environment for fibroblast proliferation in a hyperbaric oxygen environment.

PMID:
16172361
DOI:
10.1001/archotol.131.9.809
[Indexed for MEDLINE]

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