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J Cell Biol. 2005 Sep 26;170(7):1047-55. Epub 2005 Sep 19.

Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis.

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  • 1Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), 01307 Dresden, Germany. kinoshita@mpi-cbg.de

Abstract

Centrosomes act as sites of microtubule growth, but little is known about how the number and stability of microtubules emanating from a centrosome are controlled during the cell cycle. We studied the role of the TACC3-XMAP215 complex in this process by using purified proteins and Xenopus laevis egg extracts. We show that TACC3 forms a one-to-one complex with and enhances the microtubule-stabilizing activity of XMAP215 in vitro. TACC3 enhances the number of microtubules emanating from mitotic centrosomes, and its targeting to centrosomes is regulated by Aurora A-dependent phosphorylation. We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis.

PMID:
16172205
PMCID:
PMC2171544
DOI:
10.1083/jcb.200503023
[PubMed - indexed for MEDLINE]
Free PMC Article
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