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EMBO Rep. 2005 Nov;6(11):1040-4.

The c15 ring of the Spirulina platensis F-ATP synthase: F1/F0 symmetry mismatch is not obligatory.

Author information

1
Institute of Microbiology, Eidgenössische Technische Hochschule, Wolfgang-Pauli Strasse 10, Zurich 8093, Switzerland.

Abstract

The oligomeric c ring of the F-ATP synthase from the alkaliphilic cyanobacterium Spirulina platensis was isolated and characterized. Mass spectroscopy analysis indicated a mass of 8,210 Da, reflecting that of a c monomer. The mass increased by 206 Da after treatment with the c-subunit-specific inhibitor dicyclohexylcarbodiimide (DCCD), which indicated modification of the ion-binding carboxylate by DCCD. Atomic force microscopy topographs of c rings from S. platensis showed 15 symmetrically assembled subunits. The c15-mer reported here is the largest c ring that is isolated and does not show the classical c-ring mismatch to the three-fold symmetry of the F1 domain.

PMID:
16170308
PMCID:
PMC1371026
DOI:
10.1038/sj.embor.7400517
[Indexed for MEDLINE]
Free PMC Article

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